Fig. 2

miR-191 promoted the cellular proliferation of RA-FLS. a Confirmation of the level of miR-191 in RA-FLS after transfected with mimic-miR-191 or inhibitor-miR-191 for 24 h by quantitative RT-PCR. b RA-FLS transfected with mimic-miR-191 and inhibitor miR-191 were plated at 4 × 10³ cells/well in 96-well plates; the cell viability of RA-FLS was determined by CCK8 at 0, 24, 48, and 72 h. c Increased expression of cyclinD1 in RA-FLS transfected with mimic-miR-191 for 48 h. Quantitative analysis on three repeats were presented. d Decreased expression of cyclinD1 at the protein level in RA-FLS transfected with inhibitor-miR-191 for 48 h. Quantitative analysis on three repeats was presented. e The cell cycle analysis by flow cytometry indicating the increased G₁/S transition of RA-FLS by mimic-miR-191 transfection for 48 h. f The cell cycle analysis by flow cytometry indicating the decreased G₁/S transition of RA-FLS by inhibitor-miR-191 after transfection for 48 h. All experiments were repeated three times. Data were presented as mean ± SEM (n = 3, ***p < 0.001, *p < 0.05 by t test)