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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: MicroRNA-143-5p targeting eEF2 gene mediates intervertebral disc degeneration through the AMPK signaling pathway

Fig. 4

IDD rats exhibit upregulated miR-143-5p and activated AMPK signaling pathway yet inhibited NP cell differentiation and eEF2. a miR-143-5p expression and mRNA expression of Bax, AMPK, mTOR, Bcl-2, cyclinD, and eEF2 in normal IVD and degenerative IVD. b, c Protein levels and bands of Bax, AMPK, mTOR, Bcl-2, cyclinD, and eEF2 in normal IVD and degenerative IVD. d Protein levels of COL2, ACAN, and DCN in normal IVD and degenerative IVD; the experiment was repeated three times independently; data were expressed by mean ± standard deviation; comparison of data between two groups was conducted using a t-test; *, p < 0.05 vs. normal IVD; miR-143-5p, microRNA-143-5p; IVD, intervertebral disc; eEF2, eukaryotic translation elongation factor 2; AMPK, AMP activated protein kinase; Bax, Bcl-2 Associated X protein; Bcl-2, B cell lymphoma-2; mTOR, mammalian target of rapamycin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; COL2, collagen type II; ACAN, aggrecan; DCN, decorin

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