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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: MicroRNA-143-5p targeting eEF2 gene mediates intervertebral disc degeneration through the AMPK signaling pathway

Fig. 5

miR-143-5p directly targets eEF2. a Putative binding site of miR-143-5p to the 3′UTR of eEF2 predicted using Targetscan. b The luciferase activity detection using vectors constructed with eEF2-3′UTR in the presence of miR-143-5p mimic or NC; three replicates were settled in each group and the experiment was repeated three times independently; data were expressed by mean ± standard deviation; comparison of data between two groups was conducted using a t-test; *, p < 0.05 vs. the NC group; miR-143-5p, microRNA-143-5p; eEF2, eukaryotic elongation factors-2; 3′UTR, 3′untranslated regions; Wt, wild-type; Mut, mutant; NC, negative control; ANOVA, analysis of variance

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