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Fig. 9 | Arthritis Research & Therapy

Fig. 9

From: MicroRNA-143-5p targeting eEF2 gene mediates intervertebral disc degeneration through the AMPK signaling pathway

Fig. 9

Inhibition of miR-143-5p suppresses NP cell apoptosis and senescence via inactivation of the AMPK signaling pathway. a The apoptosis of NP cells from degenerative IVD treated with miR-143-5p mimic, inhibitor or AICAR. b The percentage of apoptotic cells from degenerative IVD treated with miR-143-5p mimic, inhibitor or AICAR. c Senescent NP cells from degenerative IVD treated with miR-143-5p mimic, inhibitor or AICAR under an optical microscope (× 400). d SA-β-gal staining images of senescent NP cells from degenerative IVD treated with miR-143-5p mimic, inhibitor, or AICAR; the experiment was repeated three times independently; data were expressed by mean ± standard deviation; data among multiple groups were analyzed using one-way ANOVA; *, p < 0.05 vs. the control group (NP cells from normal IVD); #, p < 0.05 vs. blank and NC groups (NP cells from degenerative IVD without transfection and transfected with NC sequence, respectively); miR-143-5p mimic group refers to NP cells from degenerative IVD transfected with miR-143-5p mimic; miR-143-5p inhibitor group refers to NP cells from degenerative IVD transfected with miR-143-5p inhibitor; AICAR group refers to NP cells from degenerative IVD added with 0.5 mmol/L AICAR, AMPK signaling pathway activator; miR-143-5p inhibitor + AICAR group refers to NP cells of degenerative IVD added with 0.5 mmol/L AICAR and transfected with miR-143-5p inhibitor. NC, negative control; miR-143-5p, microRNA-143-5p; FITC, fluorescein isothiocyanate; NP, nucleus pulposus; SA-β-gal, senescence-associated β-galactosidase; ANOVA, analysis of variance

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