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Table 2 Primer sequence for RT-qPCR

From: MicroRNA-143-5p targeting eEF2 gene mediates intervertebral disc degeneration through the AMPK signaling pathway

Gene

Sequence

miR-143-5p

F: 5′-GCATCTCTGGTCAGTTGGG-3′

R: 5′-GACCTCAAGAACAGTAT-3′

eEF2

F: 5′-GAGCTCTCCGAGAACGACC-3′

R: 5′-TACAGTGCCCAGGACAGGAT-3′

mTOR

F: 5′-TTGAGGTTGCTATGACCAGAGAGAA-3′

R: 5′-TTACCAGAAAGGACACCAGCCAATG-3′

AMPK

F: 5′-ATCCGCAGAGAGATCCAGAA-3′

R: 5′-CGTCGACTCTCCTTTTCGTC-3′

Bcl-2

F: 5′-CCTGCCCCAAACAAATATGAAAAG-3′

R: 5′-TTGACCATTTGCCTGAATGTGTG-3′

cyclinD

F: 5′-TCCGCAAGCATGCACAGA-3′

R: 5′-GGTGGGTTGGAAATGAACTTCA-3′

Bax

F: 5′-CGTCTGGCCCTGTATGTCTA-3′

R: 5′-CAACCACCTCTCTGTGCAAT-3′

U6

F: 5′-CTCGCTTCGGCAGCACA-3′

R: 5′-AACGCTTCACGAATTTGCGT-3′

GAPDH

F: 5′-CGTGATCGAGGGCTGTTGG-3′

R: 5′-CTGCTTCAGTTGGCCTTTCG-3′

  1. Note: RT-qPCR reverse transcription quantitative polymerase chain reaction, miR-143-5p microRNA-143-5p, eEF2 eukaryotic translation elongation factor 2, AMPK AMP activated protein kinase, Bcl-2 B cell lymphoma-2, mTOR mammalian target of rapamycin, GAPDH glyceraldehyde-3-phosphate dehydrogenase, F forward, R reverse, Bax Bcl-2 Associated X protein