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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis

Fig. 2

The effects of HKI/II on the glycolytic activities and cell survival of RASFs. a The silencing efficiency was confirmed by Western blot in RASFs (n = 3). After transfection with siHKI, siHKII or siHKI/II (50 nM) or its control (siCtrl) for 24 h, glucose uptake (b) and HKs activity (c) were determined in RASFs (n = 3). d Glucose concentrations in the incubation media of RASFs (n = 6) were periodically monitored. e l-lactate level was measured in the supernatant of RASFs (n = 6) after indicated treatments. The viability (f) and apoptosis (g) of RASFs (n = 3) were analyzed by MTS, Annexin X staining, and trypan blue. Data represent the mean ± SE. The statistical significance of differences between siCtrl and siHKI/II group was determined. *p < 0.05, **p < 0.01 significantly different; ns insignificant difference. Asterisk in d indicates siHKI/II vs siCtrl, number sign indicates siHKI vs siCtrl, and star indicates siHK II vs siCtrl (*p < 0.05, #p < 0.05, and p < 0.05)

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