Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Antifibrotic effects of 2-carba cyclic phosphatidic acid (2ccPA) in systemic sclerosis: contribution to the novel treatment

Fig. 3

2ccPA inhibited the upregulation of profibrotic markers in normal skin fibroblasts stimulated with TGF-β1. Normal skin fibroblasts (n = 3) were incubated with or without 10 μM 2ccPA in the presence or absence of 10 ng/mL TGF-β1 for 48 h. a The expression of COL1A1, COL1A2, CTGF, and ACTA2 mRNA were determined by qPCR. b Normal skin fibroblasts (n = 3) were incubated with 10 μM 2ccPA in the presence or absence of 10 ng/mL TGF-β1 for 72 h. The protein expression of type I collagen and CCN2 in cell lysates of normal skin fibroblasts were assessed with Western blotting. Data are the representative of three independent experiments. The bars represent as median with IQR. *p < 0.05

Back to article page