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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Ipriflavone attenuates the degeneration of cartilage by blocking the Indian hedgehog pathway

Fig. 2

Chondroprotective effect of ipriflavone (IP) in human chondrocytes. a Real-time PCR results showed reduced mRNA expression of key genes in the Ihh pathway, Smo, Gli-1,Gli-2,Gli-3, and Runx-2, at 48 h after IP treatment, and among the three kinds of Glis, the reduction of Gli-2 was especially significant. The MMP-13 and type X collagen mRNA levels were decreased, and the type II collagen mRNA level was significantly increased in human chondrocytes. b Western blot results indicated that in chondrocytes, the expression of Smo and Runx-2 protein was decreased at 48 h after IP treatment, MMP-13 and type X collagen expression was decreased in the IP treatment group, and type II collagen expression was increased in the IP treatment group. The gray value of the Western blot bands was semiquantified using Image Analysis Software (Image Lab 3.0). Values are the mean ± SEM. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 versus the DMSO group

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