Fig. 1

Characteristic of the differentiated macrophages. a The rounded, non-adherent U937 cells were transformed to adherent flatten differentiated macrophages with elongated arms stimulated with 500 ng/mL phorbol 12-myristate 13-acetate (PMA) for 48 h. b The expression of CD86 was significantly increased in differentiated macrophages compared with U937 cells. c A representative example. d The addition of LPS could significantly increase the protein levels of phosphorylated p65 subunit of NF-κB (p-p65) in nuclear extract of differentiated macrophages