Fig. 2

Protein expression of PADI2, PADI4, and citrullinated histone 3 in U937 cells, differentiated macrophages, and macrophages stimulated with lipopolysaccharides (LPS). a The protein expression of PADI2 increased in differentiated macrophages compared with U937 cells as detected by Western blot analysis. The addition of LPS slightly increased the protein expression of PADI2 in macrophages. b The protein expression of PADI4 increased in differentiated macrophages compared with U937 cells. The addition of LPS did not affect the protein expression of PADI4 in macrophages. c The protein level of citrullinated histone 3 (cit-H3) slightly increased in differentiated macrophages compared with U937 cells. The addition of LPS significantly increased the protein level of cit-H3 in macrophages