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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Increased peptidylarginine deiminases expression during the macrophage differentiation and participated inflammatory responses

Fig. 6

Using siRNA to inhibit the expression of plasminogen activator inhibitor 2 (PAI-2) and its effect on cytokines expression. a The transfection of siRNA targeting PAI-2 could effectively suppress the mRNA expression of PAI-2 in U937 cells, differentiated macrophages, and macrophages stimulated with LPS. b The protein expression of PAI-2 was significantly lower in differentiated macrophages after being transfected with siRNA targeting PAI-2. c The mRNA expression levels of IL-1β, IL-6, and TNF-α in U937 cells, differentiated macrophages, and macrophages stimulated with LPS transfected with siRNA targeting PAI-2 compared with the controls. d The cytokines secretion of IL-1β, IL-6, and TNF-α in U937 cells, differentiated macrophages, and macrophages stimulated with LPS transfected with siRNA targeting PAI-2 compared with the controls. e The mRNA expression levels of IL-1β, IL-6, and TNF-α in the macrophage stimulated with LPS after transfected with of siRNA targeting PAI-2 together with plasmid encoding PAI-2 or empty plasmid. P < 0.05 compared with U937 cells. *P < 0.05 compared with differentiated macrophages

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