Skip to main content
Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: miR-449a inhibits cell proliferation, migration, and inflammation by regulating high-mobility group box protein 1 and forms a mutual inhibition loop with Yin Yang 1 in rheumatoid arthritis fibroblast-like synoviocytes

Fig. 5

miR-449a inhibits TNFα-mediated HMGB1 and YY1 overexpression and IL-6 production. RA-FLS were treated with 10 ng/ml TNFα or the bovine serum albumin negative control (Ctrl) for 48 h, HMGB1, YY1, and miR-449a expression was detected by qPCR (a, b, d), and IL-6 production was detected by ELISA (c, n = 5). After RA-FLS cells were treated with 10 ng/ml TNFα in the presence or absence of 20 nM miR-449a for 48 h, HMGB1 and YY1 expression was detected by qPCR (e, f), and IL-6 production was detected by ELISA (g, n = 5). The results shown are the mean ± SD from three independent experiments using cells from different patients. Student’s t test with Welch’s correction was used for data analysis. *p < 0.05, **p < 0.01

Back to article page