Fig. 3

ICC results for α-SMA (top row), Col-1 (middle row), and SM22 (bottom row) in control, unaffected LcSSc, affected LcSSc and affected DcSSc fibroblasts at basal levels (untreated) and after stimulation with anti-Cenp-B (ratios 1:100 and 1:200)/anti-Topo-I (ratios 1:100 and 1:200) antibodies and with SSc sera (10% v/v in DMEM). The statistics is reported with respect to the “Control.” “Control” for IgG stimulations is referred to human healthy control IgGs in a ratio 1:100 in culture medium (DMEM). “Control” for serum stimulation is referred to as human healthy control serum at 10% v/v in DMEM. Data are reported as Immunolabeling Intensity vs “Control”. Immunolabeling Intensity corresponds to the formula I × A/n where I = intensity levels, A = area, n = number of cells (*p < 0.05, **p < 0.01, ***p < 0.001)