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Table 2 Primers used for RT-qPCR

From: Antibodies against specific extractable nuclear antigens (ENAs) as diagnostic and prognostic tools and inducers of a profibrotic phenotype in cultured human skin fibroblasts: are they functional?

 

Cat. no. (Qiagen)

Forward sequence

Reverse sequence

Target gene

 ACTA2

QT00088102

CTATGCCTCTGGACGCACAACT

CAGATCCAGACGCATGATGGCA

 COL1A1

QT00037793

GATTCCCTGGACCTAAAGGTGC

AGCCTCTCCATCTTTGCCAGCA

 TAGLN

QT00072247

TCCAGGTCTGGCTGAAGAATGG

CTGCTCCATCTGCTTGAAGACC

Housekeeping gene

 GAPDH

QT00079247

GTCTCCTCTGACTTCAACAGCG

ACCACCCTGTTGCTGTAGCCAA

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Arthritis Research & Therapy

ISSN: 1478-6362

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