Fig. 4

Effects of knockdown of MGP in primary chondrocytes from patients with OA. a MGP knockdown with siRNA targeting MGP (siMGP) was carried out in cultured knee chondrocytes from nine OA patients, in comparison to the effects of a nontargeting siRNA control (siCONTROL). MGP mRNA levels were measured by qPCR. Values represent the mean ± standard deviation (SD) fold change in expression compared to siRNA control, with the data combined for the patients. b Representative results from immunoblotting demonstrating depletion of matrix Gla protein. GAPDH was used as the loading control. c Fold change in matrix Gla protein level with the immunoblotting data combined for the patients. Values represent the mean ± SD fold change compared to siRNA control. d Fold change in expression of genes following MGP knockdown, relative to that with the siRNA control. Values represent the mean ± SD fold change in expression compared to siRNA control, with the data combined for the patients. *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001