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Table 2 Associations between baseline global DNA (hydroxy)methylation and ΔDAS28 before and after 3 months of therapy

From: Higher baseline global leukocyte DNA methylation is associated with MTX non-response in early RA patients

Methylation Before MTX After MTX
   B (SE) β p B (SE) β p
1 Methylation 1.36 (0.67) 0.15 0.044 0.40 (0.55) 0.05 0.471
2 Methylation 1.41 (0.56) 0.15 0.013 0.44 (0.47) 0.06 0.385
  DAS28 − 0.51 (0.06) − 0.49 < 0.001 − 0.50 (0.06) − 0.49 < 0.001
  Erythrocyte folate (nmol/L) − 1.00 × 10−3 (2.00 × 10−4) − 0.17 0.006 − 4.00 × 10−4 (2.00 × 10−4) − 0.12 0.063
  BMI (kg/m2) 0.03 (0.02) 0.14 0.025 0.04 (0.02) 0.18 0.005
  Age (years)   –    –  
  Sex   –   0.27 (0.16) 0.11 0.098
  Smoking (current)   –   0.28 (0.16) 0.11 0.084
  ACPA status (positive)   –    –  
  Observations   181    179  
Hydroxymethylation Before MTX After MTX
B (SE) β p B (SE) β p
1 Hydroxymethylation 19.56 (18.38) 0.08 0.288 12.52 (19.26) 0.05 0.517
2 Hydroxymethylation 6.90 (15.89) 0.03 0.664 5.92 (16.75) 0.02 0.724
  DAS28 − 0.54 (0.07) − 0.52 < 0.001 − 0.52 (0.07) − 0.51 < 0.001
  Erythrocyte folate (nmol/L) − 1.00 × 10−3 (2.00 × 10−4) − 0.18 0.007 −5.00 × 10−4
(2.00 × 10−4) 		− 0.14 0.035
  BMI (kg/m2) 0.03 (0.02) 0.12 0.062 0.04 (0.02) 0.18 0.006
  Age (years) 0.01 (0.01) 0.10 0.125 0.01 (0.01) 0.13 0.057
  Sex 0.22 (0.17) 0.08 0.176 0.28 (0.16) 0.11 0.087
  Smoking (current)   –   0.28 (0.16) 0.11 0.090
  ACPA status (positive)   –    –  
  Observations   181    177  
  1. Association between mean % global DNA (hydroxy)methylation and ΔDAS28 were tested in a crude univariate model (1) and adjusted for potential confounders (2). Potential confounders were baseline DAS28 score, baseline erythrocyte folate levels (nmol/L), BMI (kg/m2), age (years), sex, smoking status (current smoker versus former + never smoker), and ACPA status. Only biomarkers that changed the association with > 10% were considered confounders. B beta coefficient, SE standard error, β standardized beta coefficients. p < 0.05 was considered significant
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Arthritis Research & Therapy

ISSN: 1478-6362

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