Fig. 2

Anti-migratory effects of syndecan-3 in vitro. a Migration of THP-1 cells to CCL7 (100 ng/ml) with increasing amounts of syndecan-3 in a Boyden chamber chemotaxis assay. The stars represent a significant ANOVA with Dunnett’s post-test (*P < 0.05, **P < 0.01, ****P < 0.0001). b The same assay was carried out using PBMCs and migration across endothelial cell-coated 96-well inserts. The stars represent a significant ANOVA with Dunnett’s post-test (**P < 0.01, ****P < 0.0001). c The 96-well format was used to help identify the functional components of syndecan-3 whereby the core protein and HS chains were added individually. Intact syndecan-3 and the syndecan-3 core protein were added at 10 μg/ml. Heparan sulfate was added at 50 and 250 μg/ml as indicated. The stars represent a significant ANOVA with Dunnett’s post-test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). For a–c, the columns represent mean values, with standard errors (n = 3 separate experiments performed in triplicates). Results are displayed as a percentage of the positive control. S3, syndecan 3; S3CP, syndecan 3 core protein; HS10, heparan sulfate at 10 μg/ml; HS250, heparan sulfate at 250 μg/ml. In all experiments, CCL7 [50 ng/ml] was added to all but the negative control (−). The positive control contained chemokine alone (+)