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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Chondrogenic induction of human osteoarthritic cartilage-derived mesenchymal stem cells activates mineralization and hypertrophic and osteogenic gene expression through a mechanomiR

Fig. 4

Gene expression of hypertrophic and osteogenic markers in OASC2 and BMSC during chondrogenic induction. a A dot graph showing quantification of RUNX2 mRNA level in the chondrogenic induction group (Chondro.) and control group (Growth) in OASC2. The expression levels of RUNX2 were significantly by chondrogenic induction at days 3 and 14. b Quantification of ADAMTS5 mRNA level in the chondrogenic induction group and control group in OASC2. The expression levels of ADAMTS5 were significantly higher than control on day 14. c Quantification of COL1A1 mRNA level in the chondrogenic induction group and control group in OASC2. The expression levels of COL1A1 were significantly higher under chondrogenic induction than control. d Quantification of OCN mRNA levels in the chondrogenic induction group and control group in OASC2. OCN levels were significantly higher under chondrogenic induction than control. e Quantification of COL10A1 mRNA level in the chondrogenic induction group and control group in OASC2. The COL10A1 expression levels were significantly higher under chondrogenic induction than control. f Quantification of ADAMTS5 mRNA level in the chondrogenic induction group and control group in BMSC. The ADAMTS5 expression level was significantly lower at day 3 after chondrogenic induction. g Quantification of COL1A1 mRNA levels in the chondrogenic induction group and control group in BMSC. There was no significant difference of COL1A1 expression levels in response to chondrogenic induction. h Quantification of COL10A1 mRNA level in the chondrogenic induction group and control group in BMSC. There was a significant increase of COL10A1 expression level at days 7 and 14 after chondrogenic induction. i Quantification of RUNX2 mRNA level in the chondrogenic induction group and control group in BMSC. Chondrogenic treatment significantly increased RUNX2 expression at day 7 after induction. j Western blot analysis showing RUNX2, COL10A1, and OCN protein expression during chondrogenic induction in OASC2. RUNX2, The COL10A1, and OCN protein levels were increased by chondrogenic induction in OASC2. Quantification of western blot is presented in Additional file 1: Figure S1b, c, d. Data are presented as mean ± SD from at least 3 sample images for each group; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001, relative to indicated control groups respectively

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