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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Chondrogenic induction of human osteoarthritic cartilage-derived mesenchymal stem cells activates mineralization and hypertrophic and osteogenic gene expression through a mechanomiR

Fig. 5

The chondrogenic, hypertrophic, and osteogenic markers expression in undifferentiated OASC2 and BMSC upon transfection of miR-365 or its inhibitor. a A dot graph showing quantification of miR-365 RNA level in the chondrogenic induction group (Chondro.) and control group (Growth) in OASC2. The expression level of miR-365 was significantly increased by chondrogenic induction at Day 14 in OASC2. b MiR-365 RNA levels in BMSC. The expression level of miR-365 was significantly increased by chondrogenic induction at Day 14 in BMSC. c, d The protein expressions of MMP13, COL10A1, ADAMTS5, SOX9, RUNX2, and β-ACTIN in OASC2 transfected with miR-365 mimic and miR-365 inhibitor; western blot images are representative of three samples (n = 3). Quantification of western blot is presented in Additional file 1: Figure S2. e MiR-365 levels were significantly increased after transfection of miR-365 (miR-365 mimic) in comparison to transfection control (Mimic Ctrl) in OASC2. f The SOX9 mRNA level was significantly increased by miR-365 transfection in OASC2. g The ACAN mRNA level was significantly increased by miR-365 transfection in OASC2. h The IHH mRNA level was significantly increased by miR-365 transfection in OASC2. i The RUNX2 mRNA level was significantly increased by miR-365 transfection in OASC2. j The ADAMTS5 mRNA level was significantly increased by miR-365 transfection in OASC2. k The COL1A1 mRNA level was significantly increased by miR-365 transfection in OASC2. l The OCN mRNA level was significantly increased by miR-365 transfection in OASC2. m MiR-365 levels were significantly increased after transfection of miR-365 (miR-365 mimic) in comparison to transfection control (Mimic Ctrl) in BMSC. n No significant change of SOX9 mRNA levels after miR-365 transfection in BMSC. o The ACAN mRNA level was significantly decreased by miR-365 transfection in BMSC. p No significant change of IHH mRNA levels after miR-365 transfection in BMSC. q The RUNX2 mRNA level was significantly increased by miR-365 transfection in BMSC. r No significant change of ADAMTS5 mRNA levels after miR-365 transfection in BMSC. s No significant change of COL1A1 mRNA levels after miR-365 transfection in BMSC. t No significant change of OCN mRNA levels after miR-365 transfection in BMSC. u MiR-365 levels were significantly decreased after transfection of miR-365 inhibitor (antagonist) in comparison to control (Inhibitor Ctrl) in OASC2. v The SOX9 mRNA level was significantly inhibited by miR-365 inhibitor in OASC2. w The ADAMTS5 mRNA level was significantly inhibited by miR-365 inhibitor in OASC2. x The RUNX2 mRNA level was significantly inhibited by miR-365 inhibitor in OASC2. y The COL1A1 mRNA level was significantly inhibited by miR-365 inhibitor in OASC2. z The OCN mRNA level was significantly inhibited by miR-365 inhibitor in OASC2. aa MiR-365 levels were significantly decreased after transfection of miR-365 inhibitor (antagonist) in comparison to control (Inhibitor Ctrl) in BMSC. ab No significant change of SOX9 mRNA levels after miR-365 inhibitor transfection in BMSC. ac No significant change of ADAMTS5 mRNA levels after miR-365 inhibitor transfection in BMSC. ad No significant change of RUNX2 mRNA levels after miR-365 inhibitor transfection in BMSC. ae The COL1A1 mRNA level was significantly inhibited by miR-365 inhibitor in BMSC. af The OCN mRNA level was significantly increased by miR-365 inhibitor in BMSC. Data are presented as mean ± SD from at least 3 sample images for each group; *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001, relative to indicated control groups respectively

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