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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: IL-1β receptor antagonist (IL-1Ra) combined with autophagy inducer (TAT-Beclin1) is an effective alternative for attenuating extracellular matrix degradation in rat and human osteoarthritis chondrocytes

Fig. 3

Effect of IL-1Ra, TAT, and IL-1Ra+TAT on articular cartilage degeneration in a rat OA model. a Rat chondrocytes pretreated with IL-1β (20 ng/ml) for 36 h were treated by IL-1β (20 ng/ml)+IL-1Ra (40 ng/ml) for 36 h, IL-1β (20 ng/ml) for 28 h followed by co-treatment with TAT (10 μM) for 8 h, or IL-1β (20 ng/ml)+IL-1Ra (40 ng/ml) for 28 h followed by co-treatment with TAT (10 μM) for 8 h, respectively. The Col II (1:2000), Aggrecan (1:1000), and β-actin (1:40,000) protein levels were detected via western blotting. b Human OA chondrocytes pretreated with IL-1β (10 ng/ml) for 24 h were treated by IL-1β (10 ng/ml)+IL-1Ra (40 ng/ml) for 24 h, IL-1β (10 ng/ml) for 16 h followed by co-treatment with TAT (10 μM) for 8 h, or IL-1β (10 ng/ml)+IL-1Ra (40 ng/ml) for 16 h followed by co-treatment with TAT (10 μM) for 8 h, respectively. The Col II (1:2000), Aggrecan (1:1000), and β-actin (1:40,000) protein levels were detected via western blotting. c, d IL-1Ra (500 μg/ml, 40 μl), TAT (1.5 mg/kg), and NS (normal saline, 0.9% NaCl) were injected intra-articularly in the knees of a rat OA model once every three days for two weeks, respectively. Representative images (left) of H.E. and Safranin O-Fast green staining and graphs (right) of the OARSI scores in three weeks post-operative groups (c). Representative images (left) of H.E. and Safranin O-Fast green staining and graphs (right) of the OARSI scores in five weeks post-operative groups (d). The values represent the means ± 95% CI (n = 6,*p < 0.05, *p < 0.05, **p < 0.01, ****p < 0.0001, original magnification× 4, × 10)

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