Fig. 2

TREM-1 expression in monocytes is enhanced co-cultured with RASF. a Monocytes were co-cultured with RASF (n = 5) with various mixing ratios for 24 h. TREM-1 expression on the CD14-gated cells was assessed using flow cytometry. b Monocytes were co-cultured with RASF (n = 5) with 100:1 ratio for various cultural time. TREM-1 level on the CD14-gated cells were detected by flow cytometry. c After monocytes were co-cultured with RASF (n = 5) or OASF (n = 5) or PTSF (n = 5) with 100:1 ratio for 24 h, TREM-1 expression in monocytes was assessed using flow cytometry. The left panel was the representative flow cytometric histograms, and the right panel was a statistical chart. d Transwell assay was performed in which monocytes were separated from allogeneic RASF; as comparison, monocytes were cultured together with RASF (n = 5). TREM-1 expression on the CD14-gated cells was shown as the mean fluorescence intensity (MFI) values. The left panel was the representative flow cytometric histograms, and the right panel was a statistical chart. *P < 0.05, by ANOVA followed by Bonferroni’s post hoc test