Fig. 5
CD14+ cells were isolated from frozen PBMC of three pSS individuals, as described in the “Methods” section. Either 2 × 105 CD14− cells/well or 4 × 104 CD14+ cells/well were incubated with indicated proteins for 16 h and 48 h, in presence or absence of 100 μM RO5459072, respectively. Subsequently, supernatants were analysed by ELISA for TNF-α (a), IL-6 (b) and IL-10 (c). Results were normalized to 4 × 104 cells/well. Values reflect mean of triplicates + standard error of the mean