Proteomic and histopathological characterisation of sicca subjects and primary Sjögren’s syndrome patients reveals promising tear, saliva and extracellular vesicle disease biomarkers

Table 4 Highly upregulated proteins in tear fluid of pSS patients

Number	Gene	Related protein*	Classification and function**
Non-SS vs. pSS
1	LMO7	LIM domain only protein 7	Ubiquitination, cell signalling, cell adhesion
2	HUWE1	E3 ubiquitin-protein ligase HUWE1	Mediates ubiquitination, neutrophil degranulation, cell differentiation
3	TPD52	Tumour protein D52	B cell differentiation, cell proliferation, Ca²⁺-signalling
Controls vs. pSS
1	LMO7	LIM domain only protein 7	Ubiquitination, cell signalling, cell adhesion
2	HUWE1	E3 ubiquitin-protein ligase HUWE1	Mediates ubiquitination, neutrophil degranulation, cell differentiation
3	TPD52	Tumour protein D52	B cell differentiation, cell proliferation, Ca²⁺-signalling

*The three most upregulated immunological proteins in whole saliva of pSS patients deviating in replicates, i.e. number of individuals (frequency), and spectral counts, as identified by proteomics analysis and Scaffold (v 4.4.6, http://www.proteomesoftware.com/products/scaffold/)
**The classification and functions of the proteins presented were identified using publicly available databases, such as UniProt (http://www.uniprot.org)

ISSN: 1478-6362