Fig. 5

a–d Quantification of gene expression (arbitrary units) of Redd1, Foxo1, Trim63 and Fbxo32, determined by RT qPCR, in ex vivo quadriceps biopsies. e–h Representative images of primary murine myoblast and myotubes and quantification of gene expression of Redd1, Foxo1, Trim63 and Fbxo32, determined by RT qPCR and j representative western blot staining for p-Foxo1, total Foxo1, pEF2 and p-EF2 after loading of 20 μg of protein and normalisation to ponso staining in quadriceps for either WT mice and TNF-Tg animals receiving either vehicle or corticosterone (100 μg/mL) in drinking water over 3 weeks or in primary myotubes treated with either vehicle, TNFα (10 ng/ml), corticosterone (1 μmol/l) or a combination of TNF and corticosterone for 48 h. Values are expressed as mean ± standard error of six animals or primary cultures derived from four separate animals. Statistical significance was determined using two-way ANOVA with a Tukey post hoc analysis. *P < 0.05, **P < 0.005, ***P < 0.001