Fig. 1

Effects of curcumin on MSU-induced degradation of IκBα and NF-κB activation in THP-1 cells. a THP-1 cells were pretreated with curcumin for 1 h and then stimulated with MSU suspensions. Total protein extracted from THP-1 cells was reacted with anti-IκBα by western blotting. b Densitometry analysis of IκBα. c THP-1 cells were pretreated with curcumin for 1 h and then stimulated with MSU suspensions. Cytosolic and nuclear protein extracted from THP-1 cells was respectively reacted with anti-phosphorylated P50 (p-P50), anti-phosphorylated P65 (p-P65), anti-P50, and anti-P65 by western blotting. c–i Densitometry analysis of p-P50, p-P65, P50, and P65 protein. The data represent the mean ± SEM for three experiments