Fig. 8

Curcumin blocks the decrease of mitochondrial membrane potential and reduces the production of mitochondrial ROS in the MSU-stimulated THP-1 cells. a Representative micrographs of MitoTracker Red- and MitoTracker Green-labeled THP-1 cells in the absence (Control) or presence of MSU (0.2 mg/ml) or combinations (0.2 mg/ml MSU + 5 μM curcumin); blue shows nuclei staining with Hoechst 33342. Scale bar: 10 μm. b The relative fluorescence intensities of MitoTracker Green were quantified in MSU-treated THP-1 cells which were divided by the fluorescence intensities of MitoTracker Green in the MSU-untreated THP-1 cells. c The relative fluorescence intensities of MitoTracker Red were quantified in MSU-treated THP-1 cells which were divided by the fluorescence intensities of MitoTracker Green in the MSU-untreated THP-1 cells. d The ratio of relative fluorescence intensities of MitoTracker Red to the relative fluorescence intensities of MitoTracker Green. e Representative micrographs of MitoSOX Red- and MitoTracker Green-labeled THP-1 cells in the absence (Control) or presence of MSU (0.2 mg/ml) or combinations (0.2 mg/ml MSU + 5 μM curcumin). f The relative fluorescence intensities of MitoSOX Red were quantified in MSU-treated THP-1 cells which were divided by the fluorescence intensities of MitoSOX Red in the MSU-untreated THP-1 cells; blue shows nuclei staining with Hoechst 33342. For all measurements, the values were from three independent experiments with four fields of view in each experiment