Fig. 1

Expression of lineage-associated transcription factors and cytokines in ILC subsets from wild-type mice. a Gating strategy for ILC1s (NK1.1+), ILC2s (ST2+), CCR6+ ILC3s (CCR6+), and NKp46+ ILCs (NKp46+). ILCs were defined as CD45+, Lineage−, CD90+, and CD127+. b, c Quantitative real-time-PCR analysis of lineage-affiliated transcription factors (b) and cytokines (c) in purified ILC subpopulations. Each ILC subset was obtained from the spleen cells of two to three mice. Results are expressed as means ± SD in triplicate samples from two independent experiments, each using two to three mice, and were evaluated using an unpaired Student’s t test. N.S., not significant; N.D., not detected