Fig. 5From: Long non-coding RNA HOTAIR modulates intervertebral disc degenerative changes via Wnt/β-catenin pathwayHOTAIR activates the Wnt/β-catenin pathway in human NP cells. a The mRNA expression of Wnt1 and β-catenin in NP cells was analyzed by qRT-PCR. b–d The protein expression levels of Wnt1 and β-catenin in NP cells were analyzed by western blot. e β-catenin expression was determined by immunohistochemistry staining in NP cells. Scale bar = 100 μm. Data are presented as mean ± SD from 3 samples for each group in 3 independent experiments. Data were analyzed using Student’s t test. *P < 0.05 vs. vector groupBack to article page