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Table 1 The primer sequence for RT-qPCR

From: Long non-coding XIST raises methylation of TIMP-3 promoter to regulate collagen degradation in osteoarthritic chondrocytes after tibial plateau fracture

GenePrimer sequence (5′–3′)
LncRNA XISTF: 5′-GACACAAGGCCAACGACCTA-3′
R: 5′-TCGCTTGGGTCCTCTATCCA-3′
TIMP-3F: 5′-ATCTCCCAGACCCTCTTCCC-3′
R: 5′-AGTCTGGTTGGGACATGCAG-3′
GAPDHF: 5′-CCATGTTCGTCATGGGTGTGAACCA-3′
R: 5′-GCCAGTAGAGGCAGGGATGATGTTC-3′
  1. Notes: F forward, R reverse, LncRNA long noncoding RNA, XIST X-inactive specific transcript, TIMP-3 tissue inhibitor of metalloproteinase 3, GAPDH glyceraldehyde phosphate dehydrogenase, RT-qPCR reverse transcription quantitative polymerase chain reaction