Skip to main content

Table 1 The primer sequence for RT-qPCR

From: Long non-coding XIST raises methylation of TIMP-3 promoter to regulate collagen degradation in osteoarthritic chondrocytes after tibial plateau fracture

Gene

Primer sequence (5′–3′)

LncRNA XIST

F: 5′-GACACAAGGCCAACGACCTA-3′

R: 5′-TCGCTTGGGTCCTCTATCCA-3′

TIMP-3

F: 5′-ATCTCCCAGACCCTCTTCCC-3′

R: 5′-AGTCTGGTTGGGACATGCAG-3′

GAPDH

F: 5′-CCATGTTCGTCATGGGTGTGAACCA-3′

R: 5′-GCCAGTAGAGGCAGGGATGATGTTC-3′

  1. Notes: F forward, R reverse, LncRNA long noncoding RNA, XIST X-inactive specific transcript, TIMP-3 tissue inhibitor of metalloproteinase 3, GAPDH glyceraldehyde phosphate dehydrogenase, RT-qPCR reverse transcription quantitative polymerase chain reaction