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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: iNOS dependent and independent phases of lymph node expansion in mice with TNF-induced inflammatory-erosive arthritis

Fig. 1

iNOS dependent and independent phases of lymph node expansion in TNF-Tg mice. Female (ac) and male (df) mice with the indicated genotype were studied to determine body weight (a, d), PLN volume via ultrasound (b, e), and blood flow within the PLN (c, f) as described in the “Methods” section, and the data are presented for each mouse with mean ± SD for the group. Genetic iNOS ablation had no effect on weight in female mice (a). Popliteal lymph node volume was initially increased in both TNF-Tg and iNOS−/−× TNF-Tg at 2 months of age in female mice compared to WT and iNOS−/− littermates (b). However, at 3 months of age, TNF-Tg PLN volume continues to increase while iNOS−/−× TNF-Tg does not, suggesting an iNOS dependent and independent phase of lymph node expansion. Interestingly, there were no significant differences in NPDV over time (c). These findings are conserved in male mice such that iNOS−/− does not significantly protect the mice from weight loss, and there are iNOS dependent and independent phases of PLN expansion (df). Statistical analysis: n = 4–6 mice per group, n = 8–12 PLNs, three-way mixed model, *p < 0.05, **p < 0.01, p < 0.001

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