Fig. 2

Genetic iNOS ablation preserves lymphatic contractility in TNF-Tg mice. NIR-ICG imaging was performed on female (a) and male (b) mice with the indicated genotype to determine LV contraction frequency as described in the “Methods” section, and the data are presented for each mouse with mean ± SD for the group. There was no effect on lymphatic contractility in female mice at 2 or 3 months of age; however, at 4 months of age, iNOS^−/−× TNF-Tg mice had significantly increased lymphatic contractions compared to TNF-Tg mice (a). In male mice, TNF-Tg mice had significantly decreased contractions at 5 months of age compared to WT mice, while contractions in iNOS^−/−× TNF-Tg mice were preserved. Statistical analysis: n = 3–10 per group, three-way Mixed Model, *p < 0.05, **p < 0.01, ***p < 0.001