Fig. 1
Activity of Wnt/β-catenin signaling in the superficial zone (SFZ) of mouse adult articular cartilage. a LacZ staining of tibial articular cartilage of 12-week-old TOPGAL and WT mice. Scale bars, 20 μm. b Immunostaining of lubricin (Prg4) in tibial articular cartilage of 12-week-old WT mice. Scale bar, 20 μm. Red arrowheads indicate positive areas. c Bright field and fluorescent images of a knee joint of 12-week-old Prg4-CreERT2;Ai14 mouse 3 days after tamoxifen treatment. Scale bars, 20 μm. d Immunostaining of red fluorescent protein (RFP) in tibial articular cartilage of 12-week-old Prg4-CreERT2;Ai14 mouse with [TM(+)] or without tamoxifen treatment [TM(−)]. Scale bar, 20 μm