Fig. 4

Regulation of Prg4 expression by Wnt/β-catenin signaling. a–c Protein levels of Ctnbn1 and mRNA levels of Prg4, Acan, and Ctnnb1 in SFZ cells derived from a Prg4-Cre^ERT2;Ctnnb1^fl/fl mice treated with 4-hydroxytamoxifen (4OHT) or vehicle for 48 h, b Prg4-Cre^ERT2;Ctnnb1-ex3^fl/wt mice treated with 4OHT or vehicle for 48 h, or c WT SFZ cells treated with CHIR99021 or vehicle for 48 h. d Immunostaining of Prg4 in mouse femoral heads cultured with CHIR99021 or vehicle for 48 h. Scale bars, 20 μm. All data are expressed as mean ± SD of three biologically independent samples per group. *P < 0.05 versus vehicle (Student’s unpaired two-tailed t test)