Fig. 2

Renal absorption of LNA-anti-miR-150 and the suppression of renal miR-150. a Renal absorption of LNA-anti-miR-150 was detected by fluorescence of FAM-labeled LNA-anti-miR-150 at 6 h after the subcutaneous injection of 2.0 mg/kg and 4.0 mg/kg to six mice per group. Open squares represent glomeruli, white arrows represent tubules, and red arrows represent vessels (magnification × 1.5, bar = 2000 μm; magnification × 100, bar = 25 μm; magnification × 400, bar = 100 μm). b, c The levels of renal miR-150 were measured by qPCR 6 h after one dose of 2.0 mg/kg or 4 mg/kg of LNA-anti-miR-150 subcutaneous injection and d 8 weeks after LNA-anti-miR-150 (2 mg/kg, twice weekly for total 16 doses) administration. Data are expressed as mean ± SD from six mice per group. Statistical significance was determined using two-way ANOVA (^#p < 0.05, LN vs. WT; *p < 0.05, LNA-anti-miR-150 vs. the scrambled LNA)