Fig. 6

The effect of LNA-anti-miR-150 on renal pro-inflammatory cytokines and macrophages in LN mice. a The renal mRNA levels of cytokines including Ifnγ, Il6, and Tnf determined by qPCR; b renal protein levels of F4/80, a macrophage biomarker, examined by western blotting and the density of the blots; and c immunoflurescent staining of CD68, an alternative macrophage biomarker, and its integrated optical density per area (IOD/area) in glomeruli (magnification × 400, bar = 30 μm) and the outer medulla (magnification × 200, bar = 100 μm) of paraffin-embedded kidney sections taken from WT and LN mice treated with the scrambled LNA or LNA-anti-miR-150 (subcutaneous injection twice weekly for 8 weeks). Data are expressed as mean ± SD from six mice per group. Statistical significance was determined using two-way ANOVA in a and t test in b, c (^#p < 0.05, LN vs. WT; *p < 0.05, LNA-anti-miR-150 vs. the scrambled LNA)