Fig. 3

Inflammation, thermal sensitivity, and locomotion analyses in untreated and MTX-treated CIA mice. a Relative skin temperature in complete Freund’s adjuvant (CFA) (n = 28 paws, green circles), untreated collagen-induced arthritis (CIA) (n = 40 paws, red triangles), methotrexate (MTX) poor responder (n = 14 paws, white squares), MTX mild responder (n = 7 paws, dashed squares), and MTX high responder mice (n = 23 paws, black squares). b, Relative latency before paw withdrawal (thermal hyperalgesia) in CFA (n = 26 paws, green circles), untreated CIA (n = 40 paws, red triangles), MTX poor responder (n = 13 paws, white squares), MTX mild responder (n = 7 paws, dashed squares), and MTX high responder mice (n = 13 paws, black squares). c Relative distance traveled (open field test) by CFA (n = 14, green circles), untreated CIA (n = 20, red triangles), MTX poor responder (n = 6, white squares), MTX mild responder (n = 6, dashed squares), and MTX high responder mice (n = 11, black squares) in 10 min. d Relative print area of hind paws (Catwalk™) of CFA (n = 6, green circles), untreated CIA (n = 13, red triangles), MTX poor responder (n = 8, white squares), MTX mild responder (n = 5, dashed squares), and MTX high responder mice (n = 5, black squares). The asterisk symbol is used for comparison with the CFA group, and the dollar sign for comparison with the MTX high responder group; *^,$p < 0.05, **^,$$p < 0.01, ***^,$$$p < 0.001