Fig. 6

Response of IFP and SCAT preadipocytes to stimulation by conditioned media of the synovium from OA patients. Preadipocytes isolated from the IFPs and SCATs of OA patients (n = 4 to 5) were stimulated or not (Ctrl) with conditioned media from the inflamed part of synovium from two OA patients (Syn#1 and Syn#2) before the RT-qPCR analysis of the mRNA expression of IL-6 (a), IL-8 (b), Cox2 (c), MMP-1 (d), and MMP-3 (e). The relative mRNA expression was normalized to that of 18S RNA. Data are expressed as the relative expression of the genes of interest to control unstimulated cells. Error bars are SD. Statistical analysis compared the effect of stimulation by conditioned media from inflamed OA synovium to control unstimulated preadipocytes, using the non-parametric Friedman and Dunn’s multiple comparison tests. The effect of Syn#1 and Syn#2 conditioned media on IFP-derived preadipocytes relative to SCAT-derived preadipocytes was assessed using the non-parametric non-paired Mann-Whitney test