Protein profiling and network enrichment analysis in individuals before and after the onset of rheumatoid arthritis

Table 1 The ten proteins with the highest significance using multifactorial linear regression for pre-symptomatic individuals, RA patients, and controls compared two-by-two

Pre-symptomatic individuals vs. controls
Protein	p value	Up or downregulated^a
TNF	1.94E−07	↑
PRR16	2.68E−07	↑
CSF2	2.05E−06	↑
CCDC85C	2.91E−06	↑
CASP8	3.72E−06	↑
IL33^†	5.45E−06	↑
FAM81A	5.77E−06	↑
SELE	8.44E−06	↑
HTRA1	1.39E−05	↑
MMP10	2.16E−05	↑
Patients vs. controls
Protein	p value	Up or downregulated^a
TNF	5.52E−26	↑
PRR16	9.82E−26	↑
S100A12	1.06E−24	↑
CSF2	3.33E−24	↑
CASP8	2.35E−23	↑
FAM81A	6.74E−22	↑
MMP10	1.56E−21	↑
HTRA1	2.05E−20	↑
SELE	2.30E−20	↑
ORM1, ORM2^†	5.80E−20	↑
Pre-symptomatic individuals vs. patients
Protein	p value	Up or downregulated^b
KCNB2^†	2.92E−04	↓
S100A12	7.41E−04	↑
EPB41L5^†	1.97E−03	↑
COL6A1	2.55E−03	↓
ZNF618^†	3.82E−03	↑
S100A12	4.32E−03	↑
TGFB3^†	4.43E−03	↓
CCDC85C	6.28E−03	↑
CSF2	6.73E−03	↑
DSC3^†	6.90E−03	↑
SLC11A1^†	8.33E−03	↑

^†Protein included uniquely in one of three top ten protein lists. An expression change based comparison in ^apre-symptomatic individuals or RA patients vs. controls and ^bRA patients vs. pre-symptomatic individuals
CASP8 caspase 8; CCDC85C coiled-coil domain containing 85C; COL6A1 collagen type VI alpha 1 chain; CSF2 colony-stimulating factor 2; DSC3 desmocollin 3; EPB41L5 erythrocyte membrane protein band 4.1 like 5; FAM81A family with sequence similarity 81 member A; HTRA1 HtrA serine peptidase 1; IL33 interleukin 33; KCNB2 potassium voltage-gated channel subfamily B member 2; MMP10 matrix metallopeptidase 10; ORM1, ORM2 orosomucoid 1, orosomucoid 2; PRR16 proline rich 16; S100A12 S100 calcium-binding protein A12; SELE selectin E; SLC11A1 solute carrier family 11 member 1; TGFB3 transforming growth factor beta 3; TNF tumor necrosis factor; ZNF618 zinc finger protein 618

ISSN: 1478-6362