Fig. 3

Immunohistology of brain microglia and myeloid cells. a Representative images of Iba-1 staining in the hippocampus of lupus model mice and their controls. b Representative images of Iba-1 staining in the choroid plexus (lateral ventricles) of lupus model mice and their controls. c Comparison of the numbers of Iba-1-positive cells in the hippocampus and choroid plexus of lupus model mice and their controls. d CD11b⁺ cells accumulated in the choroid plexus of FcγRIIB^−/−Yaa mice. Among them, CD11b⁺CD11c⁺ were increased. Areas surrounded by the blue dashed lines are the dorsal third ventricles and the choroid plexus. In a, b, and c, n = 5 for FcγRIIB^−/−Yaa mice and controls, n = 3 for NZB/NZW mice and controls, n = 3 for MRL/lpr mice and controls. In d, n = 3 for FcγRIIB^−/−Yaa mice and controls. In c, numbers of Iba-1⁺ cells per designated area of the hippocampus or choroid plexus were counted. Symbols represent individual mice, and horizontal lines indicate the mean and SEM. **P < 0.01, and ***P < 0.01 by Student’s t test