Fig. 2

DZ2002 inhibited TGF-β dependent activation collagen accumulation of BJ cells. a Survival rate of BJ cells at different concentrations of DZ2002. b, c Col1a2, MMP-1, and CTGF mRNAs in the BJ cells were assessed by quantitative real-time reverse transcription-PCR. d Itgav, Itgb3, and Itgb5 mRNAs, encoding integrin αV, integrin β3, and integrin β5, were determined by quantitative real-time reverse transcription-PCR in BJ cells. e Immunofluorescence staining of α-SMA (green) in BJ cells (DAPI, blue, 24 h). f Western blot of α-SMA in BJ cells (24 h). g Immunofluorescence staining of p-Smad3 (green) in BJ cells (DAPI, blue, 2 h). h Western blot of p-Smad3 in BJ cells (2 h). e–h TGF-β = 25 ng/ml, DZ2002 = 200 μM. Mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. ns no significance