Fig. 3

DZ2002 inhibited the activation of T cells and infiltration of neutrophils and macrophages in BLM-induced mice. a TNF-α, IFN-γ, IL-1β, IL-4, IL-6, IL-10, IL-17A, and MCP-1 mRNAs were determined by quantitative real-time reverse transcription-PCR in the BLM-induced SSc mice skin single-cell suspension. b Determination of TNF-α, IFN-γ, IL-1β, IL-4, IL-6, IL-10, IL-12, and IL-17A proteins in skin tissue homogenate supernatant of BLM-induced SSc mice by ELISA (n = 7 per group). c Immunofluorescence assay for CD3⁺ and CD11b⁺ cells in skin frozen sections of BLM-induced SSc mice. d Left: The percentages of CD3⁺ CD4⁺ cells and CD4⁺ CD25⁺ cells were measured by flow cytometry; Right: Percentage statistics of CD3⁺ CD4⁺ cells and CD4⁺ CD25⁺ cells (2 groups and n = 5 per group). e Left: Representative staining analysis of intracellular IL-17, IL-4, and IFN-γ in CD4⁺ T cells by flow cytometry; Right: Percentage statistics of IL-17A⁺ CD4⁺ cells, IL-4⁺ CD4⁺, and IFN-γ⁺ CD4⁺ cells (2 groups and n = 5 per group). f Left: The percentages of neutrophils (CD11b⁺ Gr-1⁺) and macrophages (CD11b⁺ F4/80⁺) were measured by flow cytometry; percentage statistics of CD11b⁺ Gr-1⁺ cells and CD11b⁺ F4/80⁺ cells (2 groups and n = 5 per group). Mean ± SEM, PNS prednisolone. *P < 0.05, **P < 0.01, ***P < 0.001, ns no significance