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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: The protective role of the 3-mercaptopyruvate sulfurtransferase (3-MST)-hydrogen sulfide (H2S) pathway against experimental osteoarthritis

Fig. 2

3-MST deficiency exacerbates joint calcification and cartilage damage in experimental OA. a Representative immunohistochemical analysis of 3-MST expression in the knee section from sham-operated and MNX WT mice. A knee section from a sham-operated 3-Mst KO mouse was used to prove the specificity of the 3-MST antibody. Scale bars 150 μm. b Representative micro-CT scan images of WT and 3-MST KO MNX murine knee joints two months after surgery. White arrows show calcified periarticular deposits in MNX WT knees and their exacerbation in 3-MST KO mice. Graphs show CTAnalyzer quantitative analysis of new formation volume (mm3) and new formation crystal content (μg) in WT and 3-MST KO MNX knees. c Representative histologies of WT and 3-MST KO MNX knees, stained with Safranin-O. Black arrows show increased cartilage degradation in 3-MST KO mice. Scale bars 150 μm. Graphs show femoral scoring of cartilage damage and Safranin-O loss, accordingly to OARSI method. d Thiosulfate measurement in the serum of WT and 3-MST KO mice. Mice number WT n = 8, 3-MST KO n = 8

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