Fig. 1

HMGB1 induced pro-fibrotic changes and α2AP production through RAGE in fibroblasts. a The dermal fibroblasts were stimulated by HMGB1 (100, 200 ng/ml) for 24 h. The expression of each protein was examined by a Western blot analysis. The histogram shows quantitative representations of each protein (n = 3). b The dermal fibroblasts were pretreated by FPS-ZM1 (100 μM) for 30 min and then stimulated by HMGB1 (200 ng/ml) for 24 h. The expression of each protein was examined by a Western blot analysis. c The dermal fibroblasts were transfected with control or RAGE siRNA. At 48 h after transfection, the cells were used for experiments. d The siRNA-transfected dermal fibroblasts were stimulated by HMGB1 (200 ng/ml) for 24 h. The expression of each protein was examined by a Western blot analysis. The histogram shows quantitative representations of each protein (n = 3). The data represent the mean ± SEM. *P < 0.01, **P < 0.05