Fig. 2

IFN-λ2, IFN-α, IL-3, GM-CSF, and IL-6 increase IFN-λ1/3 production by pDCs stimulated with RNA-IC. The levels of IFN-λ1/3 in supernatants from healthy donor pDCs stimulated with RNA-IC for 20 h in the presence of a IFN- λ2, b IFN-α2b, c IL-3, d GM-CSF, or e IL-6 in indicated concentrations. No IFN production was detected in the absence of RNA-IC. a Cross reactivity of IFN-λ2 in the IFN-λ1/3 immunoassay was corrected for as described in additional file 1. Graphs show means with SEM based on 5–6 donors in two independent experiments per cytokine. Friedman’s test. *p < 0.05, **p < 0.01, ***p < 0.0005, ****p < 0.0001