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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Asiatic acid attenuates hypertrophic and fibrotic differentiation of articular chondrocytes via AMPK/PI3K/AKT signaling pathway

Fig. 2

Effects of AA on hypertrophic marker expression of chondrocytes. The cells were treated with or without AA (5 μM) for 3 days. a Representative immunofluorescence images of Runx2 (green), COL-X (red), and MMP-13 (yellow). Nucleus was stained with DAPI (blue). b The RFU analysis of Runx2, COL-X, and MMP-13. Unpaired t test, **P < 0.01, ***P < 0.001. c Relative mRNA expression of Runx2, COL10a1, and MMP-13. Unpaired t test, **P < 0.01. ns, not significant. d Representative western blot of Runx2, COL-X, and MMP-13. GAPDH was served as a loading control. e Representative ALP staining of chondrocytes. f The OD value analysis of ALP. Unpaired t test, *P < 0.05. Each experiment was repeated three times

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