Fig. 3

Effects of AA on fibrotic marker expression of chondrocytes. The cells were treated with or without AA (5 μM) for 3 days. a Representative immunofluorescence images of α-SMA (yellow) and COL-Ι (green). Nucleus was stained with DAPI (blue). b The RFU analysis of α-SMA and COL-Ι. Unpaired t test, *P < 0.05. c Relative mRNA expression of α-SMA and COL1a1. Unpaired t test, *P < 0.05. d Representative western blot of α-SMA and COL-Ι. GAPDH was served as a loading control. Each experiment was repeated three times