Skip to main content
Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: The effect of nicotinamide adenine dinucleotide phosphate oxidase 4 on migration and invasion of fibroblast-like synoviocytes in rheumatoid arthritis

Fig. 3

Effects of IL-17 and TNF-α treatment on NOX isoforms in RA and OA FLS. a, b RA and OA FLS were stimulated with or without IL-17 (10 ng/ml) and TNF-α (10 ng/ml) for 1 h, and then target mRNA levels were assessed by real-time PCR. GAPDH was used as a control. Data represented as fold change compared to control, respectively. Each symbol represents an individual donor. The bar represents the mean. *p < 0.05. c, d RA FLS were incubated with or without IL-17 (10 ng/ml) and TNF-α (10 ng/ml), and then mRNA expression of NOX4 was analyzed by RT-PCR at the indicated time points. e Protein levels of NOX4 were assessed by western blot at the indicated time points following treatment with IL-17 (10 ng/ml) and TNF-α (10 ng/ml). GAPDH or β-actin was used as loading control. Data represent one experiment, which was performed in triplicate with similar results

Back to article page