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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: The effect of nicotinamide adenine dinucleotide phosphate oxidase 4 on migration and invasion of fibroblast-like synoviocytes in rheumatoid arthritis

Fig. 4

IL-17- and TNF-α-induced ROS and VCAM1 are downregulated by NOX4-specific inhibition in RA FLS. RA FLS were pre-incubated with 40 μM of NOX4 inhibitor GLX351322 for 1 h, and then cells were stimulated with or without IL-17 (10 ng/ml) and TNF-α (10 ng/ml) for 1 h. a After 23 h, mitochondria-specific ROS was detected by MitoSox dye. The unstimulated control is shown as a dotted line, and cytokine stimulation is shown as a bold line. The gray-shaded area indicates treatment with both NOX4 inhibitor and cytokines. b ROS levels were quantitated in cells incubated with increasing amounts of NOX4 inhibitor and then stimulated with or without IL-17 and TNF-α. Data are shown as the mean fluorescence intensity (M.F.I.) ± S.E.M. *p < 0.05. c Flow cytometry analysis was performed using the indicated antibodies. Data represent one experiment, which was performed in triplicate with similar results

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