Skip to main content

Table 3 Short-term stability of samples. Stability of prepared samples stored in autosampler (10 °C), and stability of patient samples at RT and following repeated freeze-thawing

From: Measurement of hydroxychloroquine in blood from SLE patients using LC-HRMS—evaluation of whole blood, plasma, and serum as sample matrices

 

QCL (25 ng/mL) (n = 5)

QCH (4556.3 ng/mL) (n = 5)

Patient samples (n = 7)

CV%

Bias%

CV%

Bias%

CV%

Diff%

Autosampler, 48 h

5.2

3.8

4.0

− 2.5

Autosampler, 72 h

7.8

13.7

4.3

− 2.5

Room temp., 4 h

4.1

5.0

Freeze-thaw, 4 cycles

2.5

− 0.2

  1. Bias% percent difference from nominal conc., Diff% average percent difference, measured conc. after storage subtracted from conc. prior