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Table 6 Reproducibility testing. To evaluate the reproducibility of the protocol used, eight patient samples were extracted and analyzed at two separate occasions for all three matrices. Five QC samples (QCL and QCH) were prepared on both occasions. The reproducibility of the QC samples was high in all matrices and in patients’ samples measuring WB

From: Measurement of hydroxychloroquine in blood from SLE patients using LC-HRMS—evaluation of whole blood, plasma, and serum as sample matrices

 

Whole blood

Serum

Plasma

Mean (ng/mL)a

915

455

384

SD (ng/mL)b

27

65

148

CV (%)c

3

14

39

QCL (25 ng/mL), CV%

4

4

5

QCH (4556.3 ng/mL), CV%

3

4

4

  1. aMean of all measurements, eight samples measured twice
  2. bMean of the SD from comparing two measurements of the same sample, reflects reproducibility
  3. cMean of the CV from comparing two measurements of the same sample, reflects reproducibility